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Genetic fingerprint : ウィキペディア英語版
DNA profiling

DNA profiling (also called DNA fingerprinting, DNA testing, or DNA typing) is a forensic technique used to identify individuals by characteristics of their DNA. A DNA profile is a small set of DNA variations that is very likely to be different in all unrelated individuals, thereby being as unique to individuals as are fingerprints (hence the alternate name for the technique). DNA profiling should not be confused with full genome sequencing.〔''Kijk'' magazine, 1 January 2009〕 First developed and used in 1985,〔"The Guardian Interview With Sir Alec Jeffreys" http://www.theguardian.com/science/2009/may/24/dna-fingerprinting-alec-jeffreys〕 DNA profiling is used in, for example, parentage testing and criminal investigation, to identify a person or to place a person at a crime scene, techniques which are now employed globally in forensic science to facilitate police detective work and help clarify paternity and immigration disputes.〔(DNA pioneer's 'eureka' moment ) BBC. Retrieved 14 October 2011〕
Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic ("identical") twins.〔(【引用サイトリンク】title=Use of DNA in Identification )〕 DNA profiling uses repetitive ("repeat") sequences that are highly variable,〔 called variable number tandem repeats (VNTRs), in particular short tandem repeats (STRs). VNTR loci are very similar between closely related humans, but are so variable that unrelated individuals are extremely unlikely to have the same VNTRs.
The modern process of DNA profiling was developed in 1988.〔Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucleic Acids Research, 17, 6463-6471.〕〔Patent Jäckle H & Tautz D (1989) "Process For Analyzing Length Polymorphisms in DNA Regions" europäische Patent Nr. 0 438 512〕
==DNA profiling process==

Developed by Professor of Genetics Sir Alec Jeffreys, the process begins with a sample of an individual's DNA (typically called a "reference sample"). The most desirable method of collecting a reference sample is the use of a buccal swab, as this reduces the possibility of contamination. When this is not available (e.g. because a court order is needed but not obtainable) other methods may need to be used to collect a sample of blood, saliva, semen, or other appropriate fluid or tissue from personal items (e.g. a toothbrush, razor) or from stored samples (e.g. banked sperm or biopsy tissue). Samples obtained from blood relatives (related by birth, not marriage) can provide an indication of an individual's profile, as could human remains that had been previously profiled.
A reference sample is then analyzed to create the individual's DNA profile using one of a number of techniques, discussed below. The DNA profile is then compared against another sample to determine whether there is a genetic match.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
ウィキペディアで「DNA profiling」の詳細全文を読む



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